Journal: Poultry Science
Article Title: Research Note: Intestinal avian defensin 2 and robustness of chicks
doi: 10.1016/j.psj.2023.103175
Figure Lengend Snippet: IHC analysis of AvBD2 in cecal tonsil from a representative S chick (top) and from a representative L chick (bottom). Left panels (A, D): normal rabbit Ig used as primary antibody negative control; Right panels (B, C) and (E, F): rabbit anti-AvBD2 polyclonal antibodies; Peroxidase conjugate goat anti-rabbit Ig was used as secondary antibody. Images were analyzed by Image J (Fiji) software to quantify percent area of the tissue section stained for AvBD2 (%S+), as indicated at the top of panels B and E. V: villus; C: crypt; M: muscle; L: lumen.
Article Snippet: At 7 d of age, blood collected from ten chicks per line allowed to measure serum concentration of immunoglobulins (IgA, IgM, and IgY) by ELISA using coating (Cat no. A30-103A, A30-102A, and A30-104A) and horseradish peroxidase-conjugated (Cat no. A30-103P, A30-102P, and A30-104P) goat polyclonal anti-chicken IgA, IgM, or IgY antibodies and a chicken reference serum as a standard, all from Bethyl Laboratories (Texas).
Techniques: Negative Control, Software, Staining